KMS-12-BM

KMS-12-BM 细胞系是一种人类骨髓瘤细胞系，从一名非产生型多发性骨髓瘤患者的骨髓中提取。该细胞系代表了 B 细胞分化的未成熟浆细胞阶段，其特点是表达表面标志物 CD20、CD38 和 PCA-1，但不产生免疫球蛋白。这些细胞的显著特征是形态扭曲，许多细胞呈现多核和巨型特征。从超微结构上看，KMS-12-BM 细胞具有发达的粗面内质网和卵圆形偏心核，染色质呈周边分布，是典型的浆细胞。

KMS-12-BM细胞表现出染色体异常，尤其是t(11;14)(q13;q32)互变，这通常与多发性骨髓瘤有关。这些细胞的染色体数目范围也很广，从低二倍体到多倍体都有，表明其基因组极不稳定。与对应的 KMS-12-PE 细胞系不同，KMS-12-BM 细胞系不产生淀粉酶，缺乏免疫球蛋白分泌或表面表达，因此适用于涉及不产生免疫球蛋白的骨髓瘤的研究。此外，它在软琼脂培养条件下的克隆效率较低，菌落形成率低于 0.1%，注射到裸鼠体内没有致瘤特性。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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