B16-F10-Luc

B16-F10-Luc is a bioluminescent derivative of the B16-F10 murine melanoma cell line, which was originally established from a spontaneous melanoma arising in a C57BL/6 mouse. The parental B16-F10 line is widely used in cancer research owing to its high metastatic potential and robust, reproducible engraftment in syngeneic C57BL/6 hosts. In B16-F10-Luc cells, a luciferase gene has been stably integrated under the control of a constitutively active promoter (commonly EF-1α or CMV) by lentiviral transduction, resulting in high-level, stable luciferase expression across passages.

B16-F10-Luc cells emit bioluminescent signal in the presence of a luciferin substrate, enabling non-invasive, quantitative in vivo bioluminescence imaging (BLI) of tumour growth, dissemination and metastasis in live animals. The line is suitable for both subcutaneous and intravenous (experimental metastasis) models in C57BL/6 mice, with metastatic colonies forming preferentially in the lungs. Luminescence output is linearly proportional to viable cell number, allowing real-time, longitudinal monitoring of tumour burden without sacrifice of animals at each time point. Luciferase expression does not significantly alter the in vitro or in vivo growth kinetics of B16-F10 cells.

Key applications include evaluation of antitumour agents, immunotherapies and metastasis-inhibiting compounds in syngeneic models, as well as quantitative pharmacodynamic imaging in preclinical oncology studies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.