Jiyoye

The Jiyoye cell line is an extensively studied model derived from a human Burkitt lymphoma. Burkitt lymphoma is a type of non-Hodgkin lymphoma that predominantly affects B cells, and the Jiyoye cell line retains many of the key characteristics of this malignancy. The cells exhibit the typical chromosomal translocation between the c-MYC gene and immunoglobulin gene loci, which is a hallmark of Burkitt lymphoma. This translocation leads to the overexpression of the c-MYC oncogene, driving the proliferative and aggressive nature of the tumor cells. As such, the Jiyoye cell line is an invaluable tool for studying the molecular and genetic mechanisms underlying lymphomagenesis, especially in the context of MYC-driven cancers.

Jiyoye cells grow in suspension and are characterized by their high proliferation rate, which makes them suitable for a variety of experimental applications, including drug screening, gene expression studies, and apoptosis assays. The cell line is also frequently used in research focusing on Epstein-Barr virus (EBV), as Burkitt lymphoma cells, including Jiyoye, often harbor this virus, which is implicated in the pathogenesis of the disease. This makes Jiyoye particularly useful for investigating the interplay between viral oncogenes and cellular pathways in B cell malignancies.

Given its origin and characteristics, the Jiyoye cell line is a critical model for oncological research, particularly in understanding the pathophysiology of B cell lymphomas.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.