Nthy-ori 3-1

The Nthy-ori 3-1 cell line is a non-tumorigenic model established from normal human thyroid follicular epithelium. It was immortalized using the SV40 large T antigen (SV40T), which allows for extended culture periods without inducing transformation into a malignant phenotype. Nthy-ori 3-1 cells exhibit characteristics typical of thyroid epithelial cells, including expression of markers such as thyroglobulin and thyroid transcription factor-1 (TTF-1), which are essential for thyroid-specific functions. This cell line serves as a valuable tool for investigating normal thyroid biology, the effects of environmental toxins, and the early stages of thyroid carcinogenesis.

In research, Nthy-ori 3-1 has been used to explore the molecular mechanisms underlying thyroid disorders, including autoimmune thyroiditis and goiter. It provides a comparison to thyroid cancer cell lines, particularly in studies focused on the role of oxidative stress and DNA damage. For example, exposure of Nthy-ori 3-1 cells to genotoxic agents has been shown to induce DNA strand breaks and repair responses, which are critical for understanding mutagenesis and the development of thyroid malignancies.

Additionally, the cell line is utilized in toxicology to evaluate the impact of environmental and pharmaceutical compounds on thyroid health. Studies using Nthy-ori 3-1 cells have highlighted pathways involved in cellular response to thyrotoxic agents and endocrine disruptors. This makes Nthy-ori 3-1 a vital model for research on thyroid function regulation, disease modeling, and therapeutic screening.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.