HEK293-CLDN6

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HEK293-CLDN6 cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human claudin-6 (CLDN6), a tight junction-associated transmembrane protein belonging to the claudin family. CLDN6 is normally expressed during embryonic and fetal development but is largely absent in most healthy adult tissues, making it an attractive oncofetal antigen for targeted cancer therapy. Aberrant re-expression of CLDN6 has been identified in multiple malignancies, including ovarian cancer, testicular germ cell tumors, endometrial cancer, gastric cancer, and certain sarcomas. Stable HEK293-CLDN6 models provide a controlled system for studying CLDN6 biology and evaluating CLDN6-directed therapeutic approaches.

HEK293-CLDN6 cells are widely used in oncology research and drug development for characterization of monoclonal antibodies, antibody-drug conjugates, bispecific antibodies, CAR-T cell therapies, and other engineered immune cell platforms targeting CLDN6. The stable recombinant expression system supports quantitative assessment of antigen binding affinity, receptor density, antibody internalization, epitope specificity, and target-dependent cytotoxicity. These cells are also commonly applied in flow cytometry assay development, reporter assays, high-throughput therapeutic screening, and validation of CLDN6-targeted imaging agents. Because HEK293 cells exhibit high transfection efficiency and robust protein expression, they provide a reliable platform for recombinant membrane protein production and standardized assay generation.

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.