UWO23

The UWO23 (HPV33) cell line is derived from the tumor cells of a male patient with oral tongue cancer and is particularly notable for its expression of Human Papillomavirus type 33 (HPV33). This specific feature of UWO23 makes it a critical resource for research into the oncogenic roles of HPV in head and neck squamous cell carcinoma (HNSCC). The presence of HPV33 in these cells provides a unique opportunity to explore how this virus influences the carcinogenesis process, particularly in the context of oral and oropharyngeal regions.

Research utilizing the UWO23 cell line focuses on uncovering the molecular and genetic interactions driven by HPV33 that lead to the development and progression of cancer. This includes studying alterations in cell cycle regulation, apoptosis resistance, and changes in cellular adhesion and motility, all of which are crucial for understanding tumor behavior and metastasis. Additionally, the UWO23 cell line is instrumental in the evaluation of new pharmacological treatments and potential diagnostic biomarkers for HPV-related cancers. By elucidating the pathways through which HPV33 contributes to malignancy, researchers can develop targeted therapies that might improve therapeutic outcomes for patients suffering from HPV-associated head and neck cancers.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.