BHT101

The BHT101 cell line is derived from the lymph node metastasis of a 63-year-old woman diagnosed with anaplastic papillary thyroid carcinoma. This cell line is established from a highly aggressive and lethal form of thyroid cancer, known for its rapid progression and poor prognosis. BHT101 cells are notable for their lack of hormone production, which is typical for cells originating from anaplastic thyroid carcinoma, as these cells often lose the ability to synthesize thyroid hormones that are characteristic of more differentiated thyroid tissues.

In terms of biomarker expression, BHT101 cells are partially positive for thyroglobulin and thyroxine (T4). Thyroglobulin is a precursor glycoprotein critical for the production of the thyroid hormones T3 and T4 and is commonly used as a tumor marker in differentiating thyroid cancer types. The presence of thyroglobulin in BHT101 cells, even if only partial, is significant for research focused on thyroid cancer pathology and the molecular mechanisms underlying dedifferentiation in thyroid carcinomas. This cell line's unique profile makes it a valuable model for studying the progression and metastatic behavior of anaplastic thyroid carcinoma, providing insights into the molecular alterations that drive these processes.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.