HGC-27

HGC-27 is a human gastric carcinoma cell line derived from the metastatic site of an adult patient. The cell line exhibits an epithelial morphology and is commonly used in the study of gastric cancer pathogenesis and the cellular responses to various chemotherapy agents. HGC-27 cells have been utilized in numerous studies to investigate mechanisms of cancer cell proliferation, apoptosis, and metastasis. They serve as a valuable model for understanding the complex molecular interactions and pathways involved in gastric cancer, including the response to therapeutic compounds and the investigation of novel drug targets.

These cells are also instrumental in studying the role of various genetic and epigenetic modifications in gastric cancer progression. Research using HGC-27 has contributed to insights into cellular processes like epithelial-to-mesenchymal transition (EMT), a critical event in cancer metastasis. Additionally, the cell line has been used to explore receptor signaling pathways and their impact on cancer cell behavior, providing crucial data for the development of targeted therapies. Overall, HGC-27 is an important tool in the advancement of gastric cancer research, helping to pave the way for new therapeutic strategies and improving our understanding of disease mechanisms.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.