- Tissue
- Head and neck, oral cavity
- Disease
- Head and neck squamous cell carcinoma (HNSCC), oral cavity
Documents
UM-SCC-124
Cell line · Product number 305723
UM-SCC-124 is a human HNSCC cell line from the University of Michigan UM-SCC panel. It is part of the oral cavity SCC series characterized by exome sequen...
01General information
- Description
UM-SCC-124 is a human HNSCC cell line from the University of Michigan UM-SCC panel. It is part of the oral cavity SCC series characterized by exome sequencing in the genomic landscape study by Brenner et al. (2019). The line grows as an adherent monolayer and is used for comparative molecular and pharmacological studies within the UM-SCC oral cavity panel.
Applicable in HNSCC oral cavity research, drug sensitivity, mutational landscape analysis, radiation response, and UM-SCC multi-line panel studies.
- Tissue
- Head and neck, oral cavity
- Disease
- Head and neck squamous cell carcinoma (HNSCC), oral cavity
- Applications
- HNSCC oral cavity research; drug sensitivity; mutational landscape analysis; radiation response; UM-SCC panel studies
02Classification
- Organism
- Human
- Sex
- Unspecified
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Morphology
- Epithelial-like
- Cell type
- Epithelial cells
- Doubling time
- approx. 24 to 48 hours
04Identifiers
- Citation
- UM-SCC-124 (Cytion catalog number 305723)
- NCBI TaxID
- 9606
05Handling and culture
- Culture medium
- DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- Incubation atmosphere
37°C, 5% CO2, humidified atmosphere.
- Supplements
- Supplement the medium with 10% FBS
- Dissociation reagent
- Accutase
- Subculturing
- Remove medium, wash with PBS without calcium and magnesium, cover with Accutase, incubate 8–10 min at RT, resuspend in medium, centrifuge 300×g 3 min, discard supernatant, reseed in fresh medium.
- Split ratio
- 1 to 5
- Seeding density
- 2 to 4 × 104 cells/cm2
- Fluid renewal
- Every 2 to 3 days
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.
Follow the procedure described under Post-Thaw Recovery
06Quality control and cryopreservation
- Freeze medium
- As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
- Storage conditions
For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.
- Shipping conditions
Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
07Donor
- Donor age
- Age unspecified
- Ethnicity
- Ethnicity unspecified
08Legal and safety
- Safety precautions
- When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
- Warranty
At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.
For full details, please refer to our Warranty Statement.
- Material Transfer Agreement required
- Yes