CLS-138

CLS-138 cells were derived from the primary spindle cell sarcoma of female NMRI mice, following the induction of tumors via a single injection of Benzpyrene. This development has presented a valuable asset for the scientific community, particularly for those delving into the complexities of spindle cell sarcomas-a type of malignant tumor originating from connective tissue. The cultivation of these cells provides a unique window into understanding the pathophysiology of such tumors and exploring potential therapeutic avenues.

The introduction of CLS-138 cells into research has significantly enhanced our understanding of spindle cell sarcomas. These cells allow for a detailed examination of the molecular and genetic landscape, shedding light on the mutations and abnormalities pivotal in the oncogenesis and progression of these tumors. Through such cellular and genetic analysis, researchers can identify key drivers of disease and potential targets for therapy.

Moreover, CLS-138 cells serve as an invaluable model for testing therapeutic interventions. Exposing these cells to various treatments enables the assessment of the efficacy of numerous therapeutic agents and strategies in curbing tumor growth and inducing apoptosis. This line of investigation is crucial for the development of targeted therapies that could offer hope for better management and treatment outcomes for spindle cell sarcoma patients.

The establishment of CLS-138 cells from the spindle cell sarcomas of NMRI mice has provided researchers with a consistent and replicable model for a wide array of studies. These cells facilitate investigations into the identification of biomarkers, understanding cellular signaling pathways, and evaluating prognostic factors relevant to spindle cell sarcomas.

In essence, CLS-138 cells open new frontiers in the study of spindle cell sarcomas, offering insights into the disease's molecular underpinnings and therapeutic possibilities. Their derivation from induced tumors in NMRI mice marks a significant step forward in sarcoma research, promising advancements in treatment strategies and a deeper comprehension of this formidable cancer type.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.