FS-C57BL

FS-C57BL is a fibrosarcoma cell line derived from C57BL mice, commonly utilized in cancer research. The origin of this cell line traces back to spontaneous tumor formations in these mice, which are genetically modified to be predisposed to cancer. The FS-C57BL cell line is significant for its robust growth and reproducibility in experimental settings, making it a valuable tool for studying cancer biology, especially in the context of fibrosarcoma. The cell line exhibits characteristics typical of sarcomas, including high mitotic index and the ability to form tumors when inoculated into compatible hosts.

In research, FS-C57BL is often used to explore the cellular mechanisms underlying fibrosarcoma progression and metastasis. It serves as a model to assess the efficacy of chemotherapeutic agents and to study the genetic and molecular pathways involved in tumor growth and response to treatment. Researchers also leverage this cell line to investigate immune responses in cancer, benefiting from the C57BL mouse's well-documented immune profile. Thus, FS-C57BL aids in bridging in vitro experiments with in vivo outcomes, enhancing the translational relevance of the research conducted with these cells.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.