SNU-398

The SNU-398 cell line is derived from a hepatocellular carcinoma (HCC) of a human adult. This cell line is extensively used in liver cancer research to study the molecular mechanisms underlying hepatocarcinogenesis, tumor progression, and the development of therapeutic strategies. Hepatocellular carcinoma is a prevalent and deadly form of liver cancer, and SNU-398 cells provide a relevant model for investigating the genetic and epigenetic changes associated with this disease.

SNU-398 cells exhibit an epithelial morphology and express markers characteristic of liver cancer, such as alpha-fetoprotein (AFP) and cytokeratins. They harbor genetic mutations and alterations typical of HCC, including mutations in the TP53 gene, which is commonly associated with many cancers. Researchers utilize SNU-398 cells to explore various signaling pathways involved in liver cancer, such as the Wnt/β-catenin, PI3K/Akt, and MAPK pathways. These cells are also employed in drug screening assays to evaluate the efficacy of chemotherapeutic agents and targeted therapies, as well as in studies investigating resistance mechanisms to conventional treatments. The SNU-398 cell line's importance in hepatocellular carcinoma research lies in its ability to model liver cancer biology and to contribute to the development of more effective therapies for liver cancer patients.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.