TTA1

The TTA-1 cell line is derived from an undifferentiated thyroid carcinoma, also known as anaplastic thyroid carcinoma (ATC). This cell line exhibits the highly aggressive characteristics associated with ATC, including rapid proliferation and resistance to conventional therapies. Cytogenetic analysis of TTA-1 cells revealed extensive chromosomal abnormalities, with a modal chromosome number of 56-59 and numerous structural rearrangements. These features highlight the genetic instability typical of ATC.

TTA-1 cells have been utilized extensively in research on tumorigenicity and oncogenesis. Studies have shown that tumorigenicity of TTA-1 cells can be modulated by genetic interventions, such as the introduction of chromosome 11 through microcell-mediated chromosome transfer. The addition of this chromosome led to partial suppression of tumorigenic properties, suggesting the presence of tumor suppressor genes on chromosome 11. Such studies provide insights into potential genetic therapeutic approaches to ATC.

TTA-1 cells are known to secrete cytokines such as interleukin-6 (IL-6), which is implicated in cancer progression and the inflammatory responses associated with ATC. The production of cytokines by TTA-1 cells reflects their role in mediating tumor microenvironment interactions, making them a valuable model for studying both ATC biology and therapeutic resistance.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.