UM-SCC-127
Cell line · Product number 305725
UM-SCC-127 is a human HNSCC cell line from the University of Michigan UM-SCC panel. As part of the higher-numbered UM-SCC series, it represents one of the...
Recommended (freshest lot)
01General information
- Description
UM-SCC-127 是來自密西根大學 UM-SCC 細胞庫的人類頭頸部鱗狀細胞癌(HNSCC)細胞系。作為編號較高的 UM-SCC 系列之一,它是該細胞庫中較晚建立的細胞系之一。 該細胞系呈黏附性生長,具有上皮樣形態,可作為頭頸部鱗狀細胞癌(HNSCC)分子與藥理學比較研究的模型。
適用於頭頸部鱗狀細胞癌(HNSCC)研究、藥物敏感性研究、分子路徑分析,以及 UM-SCC 多細胞系面板實驗。適合用於藥理學檢測及異種移植模型。
- Tissue
- 頭頸部、口腔
- Disease
- 頭頸部鱗狀細胞癌(HNSCC)
- Applications
- 頭頸部鱗狀細胞癌(HNSCC)研究;藥物敏感性;分子途徑分析;UM-SCC 基因檢測面板研究;異種移植模型
02Classification
- Organism
- Human
- Sex
- Unspecified
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Morphology
- 上皮樣
- Cell type
- 上皮細胞
- Doubling time
- 約 24 至 48 小時
04Identifiers
- Citation
- UM-SCC-127(Cytion 型號 305725)
- NCBI TaxID
- 9606
05Handling and culture
- Culture medium
- RPMI 1640,含 2.0 mM 穩定谷氨醯胺,含 2.0 g/L NaHCO₃(Cytion 產品編號 820700a)
- Incubation atmosphere
37°C,5% CO₂,加濕環境。
- Supplements
- 在培養基中加入 10% 的胎牛血清(FBS)
- Dissociation reagent
- Accutase,5 分鐘,37°C
- Subculturing
- 移除培養基,用不含鈣和鎂的PBS清洗,加入Accutase覆蓋,於室溫下孵育 8–10 分鐘,重新懸浮於培養基中,以 300×g 離心 3 分鐘,棄去上清液,再將細胞接種於新鮮培養基中。
- Split ratio
- 1 至 5
- Seeding density
- 1 至 3 × 10⁴ 個細胞/cm²
- Fluid renewal
- 每 2 至 3 天
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.
Follow the procedure described under Post-Thaw Recovery
06Quality control and cryopreservation
- Freeze medium
- 作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
- Storage conditions
若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。
- Shipping conditions
冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。
07Donor
- Donor age
- 年齡不詳
- Ethnicity
- 未指定族裔
08Legal and safety
- Safety precautions
- 若計劃將低溫試管存放在液態氮中以供日後解凍,必須嚴格遵守相關安全措施。 必須穿戴適當的防護手套和防護服,且在將冷凍樣本移入或移出液氮罐時,必須佩戴口罩或安全護目鏡。此舉旨在降低因取出時冷凍試管可能發生爆炸而導致受傷的風險,此類爆炸可能造成尖銳碎片飛濺。
- Warranty
在 Cytion,我們對產品的品質與存活率提供保障。 我們保證,自本公司設施出貨後 60 天內,我們的細胞在培養開始時仍具存活能力。 我們保證所有材料均符合 分析證明書及/或產品說明書中所載的規格。
詳情請參閱我們的 《保固聲明》。
- Material Transfer Agreement required
- Yes
