Hep 3B2.1-7

Hep3B 细胞系源自一名 8 岁的肝癌患儿，是研究人类肝癌细胞及其对各种治疗药物反应的重要模型。Hep3B 细胞含有整合的乙型肝炎病毒基因组，因其独特的基因和表型特征，在研究不同药物反应方面不可或缺。

Hep 3B 人肝癌细胞系以广泛表达肝脏特异性蛋白（如甲胎蛋白（AFP）、白蛋白和其他各种标记物）而闻名，是药物代谢和肝毒性研究的宝贵工具。这种表达蛋白种类繁多，可以全面评估肝癌细胞与治疗药物的相互作用和代谢情况。

Hep 3B 细胞系及其衍生物细胞系能够追踪体内肿瘤的生长和转移，有助于研究肝癌的进展和潜在治疗方法的疗效。

Hep3B 细胞系是促进我们对肝癌生物学的了解和开发更有效的治疗策略的重要资源。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

有关详细信息，请参阅我们的 <a href="" /Who-we-are/Warranty/"" hreflang=""x-default"">保修声明 。