MLTC-1

MLTC-1 细胞系源自小鼠的莱迪格肿瘤细胞，保留了原始肿瘤对激素的反应性。这种细胞系对研究类固醇生成和莱蒂格细胞功能特别有价值。MLTC-1 细胞具有莱德细胞的主要特征，包括存在黄体生成素（LH）受体，这对刺激睾酮的产生至关重要。MLTC-1 细胞对激素治疗的反应与原始肿瘤细胞相似。用人绒毛膜促性腺激素（hCG）、黄体生成素、霍乱毒素、氟化钠和 5'-亚胺二磷酸鸟苷酯处理后，会明显刺激膜腺苷酸环化酶的活性。此外，这些细胞还能对 hCG 产生孕酮反应，这进一步突出了它们在研究激素调节和信号通路方面的作用。MLTC-1 细胞系还被用于毒理学研究，以评估各种物质对莱德细胞功能和类固醇生成的影响，因此是生殖生物学和内分泌学研究的重要工具。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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