NCI-H596

NCI-H596 细胞系来源于人类肺腺鳞癌。这种独特的细胞系被广泛用于肺癌研究，为研究腺鳞癌的特征和行为提供了一个模型，腺鳞癌是一种罕见的非小细胞肺癌亚型，同时具有腺癌和鳞癌的特征。NCI-H596细胞系对于研究这种混合型癌症的分子和遗传基础以及测试潜在的治疗干预措施非常有价值。

NCI-H596 细胞呈现上皮形态，表达腺癌和鳞癌的标志物，包括细胞角蛋白和粘蛋白。它们携带肺癌常见的基因改变，如 KRAS 和 TP53 基因突变，而这两种基因在细胞信号传导、生长和凋亡中起着关键作用。研究人员利用 NCI-H596 细胞探索肿瘤进展过程中涉及的信号通路，如表皮生长因子受体、MAPK 和 PI3K/Akt 通路。这些细胞还被用于药物发现和开发，以便对化疗药物、靶向疗法和新型治疗组合进行评估。NCI-H596 细胞系的双重组织学特征使其成为了解腺鳞癌复杂性和推进肺癌治疗策略的重要工具。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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