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SVEC4-10
Tissue
腋窝结节
Synonyms
SVEC 4-10

Documents

SVEC4-10

Cell line · Product number 305180

The SVEC4-10 cell line is derived from murine endothelial cells and is widely utilized in research focused on vascular biology and endothelial function. T...

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  • CoA
1

01General information

Description

SVEC4-10 细胞系来源于小鼠内皮细胞，被广泛用于血管生物学和内皮功能的研究。这些细胞的特点是增殖能力强，能形成毛细血管样结构，是研究血管生成和血管网络形成的极佳模型。SVEC4-10 细胞表达典型的内皮标志物，如 CD31（PECAM-1）和 von Willebrand 因子，这对它们在血管研究中的识别和功能至关重要。

除了用于血管生成研究外，SVEC4-10 细胞还被用于研究内皮细胞对各种刺激（包括细胞因子、生长因子和药剂）的反应。它们为探索内皮功能障碍的机制及其对动脉粥样硬化、高血压和糖尿病等疾病的影响提供了宝贵的体外系统。对这些细胞进行遗传操作的能力进一步提高了它们在剖析内皮细胞生物学分子通路方面的作用。总之，SVEC4-10 细胞是血管研究的重要工具，有助于了解内皮细胞的行为和病理。

Tissue
腋窝结节
Synonyms
  • SVEC 4-10

02Classification

Organism
Mouse
Sex
Male
Growth properties
Adherent
GMO status
GMO-S1
GMO status details
GMO-S1: This murine lymph node-derived endothelial-like cell line (SVEC4-10) contains an SV40 T-Antigen construct introduced by transfection, enabling immortalization of vascular endothelial cells. The insert is stably integrated. This classification applies only within Germany and may differ elsewhere.
Biosafety level
1

03Characteristics

Morphology
上皮细胞
Tumourigenic
是的，在大约 14 周的潜伏期后，这些细胞会诱发具有人类卡波西肉瘤某些组织病理学特征的纺锤形肿瘤。
Doubling time
24 至 30 小时

04Identifiers

Citation
SVEC4-10（Cytion 产品编号 305180）
NCBI TaxID
10090
Cellosaurus
CVCL_4393

05Expression and susceptibility

Receptors expressed
低密度脂蛋白（LDL）的高亲和力受体
Antigen expression
H-2 K、因子 VIII 相关抗原、VCAM

06Handling and culture

Culture medium
DMEM，w：4.5 克/升葡萄糖，w：4 毫摩尔 L-谷氨酰胺，w：3.7 克/升 NaHCO3，w：1.0 毫摩尔丙酮酸钠（Cytion 编号 820300a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基，用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS，T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞，T25 烧瓶用 1-2 毫升，T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟，使其分离。孵育后，用 10 毫升培养基轻轻混合细胞使其重悬，然后用 300xg 离心 3 分钟。弃去上清液，用新鲜培养基重悬细胞，并将其转移到已装有新鲜培养基的新烧瓶中。
Fluid renewal
每周 2 至 3 次
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Breed / subspecies
C3H/HeJ
Donor age
成人