OS-RC-2

The OS-RC-2 cell line is a human renal cell carcinoma (RCC) model established from the tumor of a Japanese male patient diagnosed with clear cell RCC. This cell line exhibits hallmark features of RCC, including the presence of numerous long microvilli on its surface and glycogen granules within its cytoplasm, as observed through electron microscopy. These characteristics align closely with the features of proximal tubular epithelial cells, thought to be the origin of clear cell RCC.

OS-RC-2 has proven to be tumorigenic in immunocompromised mice, where the histopathological features of xenograft tumors strongly resemble the original patient tumor. Chromosomal analyses of OS-RC-2 reveal a hypodiploid modal number of 40, with evidence of a marker chromosome and a specific translocation between chromosomes 2 and 13. Additionally, a large subset of the cell population exhibits a hypotetraploid karyotype with a modal number of 75. These genetic features make OS-RC-2 a valuable model for studying chromosomal aberrations and tumor biology in RCC.

Further research using OS-RC-2 has shed light on the role of cytokines in RCC, including tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Studies have demonstrated that while TNF-α does not induce DNA synthesis or cell proliferation in OS-RC-2, it can stimulate IL-6 production at high concentrations. These findings contribute to understanding the complex interplay of cytokines in RCC progression and the tumor microenvironment, making OS-RC-2 a useful tool for investigating therapeutic interventions in RCC.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.