RenCa

RenCa (Renal Carcinoma) cells are a murine renal adenocarcinoma cell line. They are derived from a tumor spontaneously developed in the kidney of a BALB/c mouse, a common inbred strain used in research. RenCa cells are used extensively to study renal cancer biology, tumor immunology, and cancer therapy, including the efficacy of immunotherapeutic agents. The cells are known for their aggressive tumor formation when implanted in syngeneic mice, making them a valuable model for in vivo experiments that aim to mimic cancer progression and metastasis in a controlled laboratory environment.

RenCa cells are characterized by a high mitotic index and are able to grow in an anchorage-independent manner, forming colonies in soft agar, which is a hallmark of oncogenic transformation. They display a fibroblast-like morphology and due to their origin in a BALB/c mouse, RenCa cells are particularly useful for research using immunocompetent mice, facilitating studies on the interaction between cancer cells and the immune system. This cell line has been utilized in numerous studies investigating the role of specific immune cells and molecules in tumor growth suppression and the potential for therapeutic intervention.

In addition to their use in immunotherapy research, RenCa cells have also served as a tool in the study of cancer metastasis mechanisms, particularly in the context of the renal system. They have been employed to assess the impact of various genes and proteins on tumor invasiveness and metastatic potential, offering insights into the pathways that might be targeted to inhibit cancer spread in renal carcinoma. These features make RenCa a crucial model in both fundamental and translational cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.