CDNR4

The CDNR4 cell line comprises a specialized subset originating from the COMMA-D cell line, known for modeling mouse mammary carcinoma. This clonal subpopulation has undergone extensive characterization, revealing a range of unique properties and functionalities. One of the most striking features of CDNR4 cells is their resemblance to mammary stem cells, which positions them as a significant resource for exploring aspects of stem cell biology, carcinogenesis, and cellular heterogeneity within populations. These cells were developed through the transfection of a transposon bearing kanamycin and neomycin resistance genes (Tn5 gene), which led to the emergence of various intriguing traits and capabilities, including their potential to differentiate into both preneoplastic and neoplastic phenotypes.

Originating from the COMMA-D line, which was initially studied for its cellular heterogeneity using a variety of techniques such as phase contrast microscopy, immunocytochemical staining, DNA content analysis, and evaluations of oncogenic potential, CDNR4 stands out as a distinct clone. Through specific transfection and selection methods, clonal subpopulations like CDNR4 were isolated, each maintaining a degree of the heterogeneity seen in the original COMMA-D parental cells. This retention of heterogeneity underscores the complex nature of these cell populations and enhances the value of CDNR4 cells in research focused on cellular differentiation and cancer progression.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.