Panc-1

PANC-1 cells, originating from a pancreatic duct carcinoma in a 56-year-old Caucasian male, stand as a pivotal epithelial cell line in the realm of cancer research, particularly in the study of pancreatic carcinoma. Panc1 cells offer a useful model for delving into the intricacies of pancreatic cancer, including ductal adenocarcinoma cell lines and their tumorigenic potential.

The cells' epithelial morphology and their capacity to exhibit diverse morphological patterns underscore their relevance in mimicking the clonal heterogeneity and complex tumor microenvironment seen in pancreatic ductal adenocarcinoma (PDAC).

PANC-1 cells express markers such as vimentin and somatostatin receptors like SSTR2, which play a crucial role in neuroendocrine differentiation. This expression profile, coupled with the cells' ability to undergo epithelial-mesenchymal transition (EMT) marker expression and EMT subtype shifting, makes them an excellent platform for exploring therapeutic strategies targeting the EMT process and neuroendocrine features of pancreatic cancer.

The cell line's karyotypic analysis reveals a hyperdiploid state with notable genetic alterations, including the loss of the Y chromosome and mutations in critical genes such as CDKN2A and the p53 gene.

In summary, PANC-1 cells provide a multifaceted model for pancreatic cancer research, enabling detailed investigations into the phenotype and genotype of pancreatic adenocarcinoma, the efficacy of targeted therapies, and the molecular mechanisms driving cancer progression.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.