HCC1143

The HCC1143 cell line is derived from a human triple-negative breast cancer (TNBC), specifically lacking estrogen receptor (ER), progesterone receptor (PR), and HER2 expression. This cell line is known for its use in modeling aggressive breast cancer phenotypes and understanding mechanisms underlying treatment resistance. HCC1143 exhibits distinct characteristics, including heterogeneity in cell subpopulations, contributing to its relevance in research focused on phenotypic plasticity and tumor cell state transitions. Studies utilizing HCC1143 have demonstrated that different cell states within the line can transition between luminal, basal, and mesenchymal differentiation states under therapeutic pressures, highlighting its role in studying therapy-induced phenotypic changes and drug resistance mechanisms.

HCC1143 cells have been used in various experimental contexts, including investigations of resistance mechanisms to chemotherapy agents like paclitaxel. Single-cell RNA sequencing (scRNA-seq) has revealed subpopulations with differential gene expression profiles linked to treatment resistance. For example, specific subpopulations such as AKR1C3+, IDO1+, and HEY1+ cells have shown increased representation following prolonged paclitaxel treatment, suggesting their role as drug-resistant phenotypes. These subtypes are associated with pathways involving reactive oxygen species (ROS), inflammatory responses, and cell cycle regulation, indicating complex adaptations that facilitate survival under chemotherapeutic stress.

Research on HCC1143 has also extended to targeted therapy studies. The application of inhibitors targeting components like ADAM-17 has shown potential in reducing the invasiveness and proliferation of this cell line, supporting its application as a model for testing novel anticancer strategies. These findings underscore HCC1143’s value for exploring both therapeutic responses and the underlying cellular dynamics that drive drug resistance in TNBC.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.