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- Tissue
- Colon
- Disease
- Adenocarcinoma, Grade IV, Dukes' type B.
- Synonyms
- SW480, SW 480, SW480E
Documents
SW-480
Cell line · Product number 300302
The SW480 cell line originated from a surgical specimen of a primary tumor of a moderately differentiated colon adenocarcinoma.
Recommended (freshest lot)
01General information
- Description
The SW480 cell line originated from a surgical specimen of a primary tumor of a moderately differentiated colon adenocarcinoma.
- Tissue
- Colon
- Disease
- Adenocarcinoma, Grade IV, Dukes' type B.
- Synonyms
- SW480
- SW 480
- SW480E
02Classification
- Organism
- Human
- Sex
- Male
- Growth properties
- Adherent
- Biosafety level
- 1
03Characteristics
- Morphology
- Epithelial-like
- Tumourigenic
- Yes, in nude mice
- Doubling time
- 20 to 25 hours
04Identifiers
- Citation
- SW-480 (Cytion catalog number 300302)
- NCBI TaxID
- 9606
- Cellosaurus
- CVCL_0546
05Expression and susceptibility
- Receptors expressed
- Epidermal growth factor (EGF), keratin (immunoperoxidase staining). Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed.
- Protein expression
- The cells express elevated levels of p53 protein.
- Antigen expression
- HLA A2, B8, B17, blood type A, Rh+. The line is negative for CSAp (CSAp-) and colon antigen 3
- Isoenzymes
- G6PD, B, PGM1, 2, PGM3, 1, 6PGD, A, PEP-D, 1, ES-D, 1
- Mutational profile
- SW-480 cells carry a homozygous Kras mutation in codon 12: GGT(Wt Gly) >GTT(Val). There is a G->A mutation in codon 273 of the p53 gene resulting in an Arg->His substitution and a C->T mutation in codon 309 resulting in a Pro->Ser substitution.
- Viruses
- Reverse transcriptase negative
- Virus susceptibility
- Human immunodeficiency virus (HIV, LAV)
- Products
- Carcinoembryonic antigen (CEA) 0.7 ng/106 cells/10 days, keratin, TGF-β. The cells have been reported to produce GM-CSF.
06Handling and culture
- Culture medium
- Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
- Incubation atmosphere
37°C, 5% CO2, humidified atmosphere.
- Supplements
- Supplement the medium with 10% FBS
- Dissociation reagent
- Accutase
- Subculturing
- Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- Seeding density
- 1 x 104 cells/cm2
- Fluid renewal
- 1 to 2 times per week
- Post-thaw recovery
- After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- Thawing and culturing
Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.
Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.
For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.
Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.
Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.
Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.
Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.
Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
07Quality control and cryopreservation
- Sterility
Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.
To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.
- Freeze medium
- As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
- Storage conditions
For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.
- Shipping conditions
Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
08Donor
- Donor age
- 50 years
- Ethnicity
- Caucasian
09Legal and safety
- Safety precautions
- When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments.
- Warranty
At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.
For full details, please refer to our Warranty Statement.
- Material Transfer Agreement required
- Yes
