OAW-28

OAW-28 is a human ovarian adenocarcinoma cell line widely utilized as a model for studying ovarian cancer, particularly epithelial ovarian adenocarcinomas, which represent the most common subtype of ovarian malignancies. OAW-28 cells provide researchers with a platform to investigate tumor biology, therapeutic resistance mechanisms, and the development of novel treatment approaches for this aggressive cancer type.

OAW-28 cells exhibit an epithelial morphology and adhere under standard cell culture conditions. These cells are characterized by their ability to represent key features of ovarian adenocarcinoma, including dysregulated cell growth, altered apoptotic pathways, and the expression of molecular markers associated with ovarian cancer progression. This cell line is also useful in exploring the genetic and epigenetic alterations commonly observed in ovarian cancers, such as mutations in TP53 or alterations in DNA repair pathways.

Due to the aggressive nature of ovarian adenocarcinoma and its frequent resistance to chemotherapeutic agents, OAW-28 is often employed in preclinical drug development. Researchers use this cell line for testing cytotoxic compounds, targeted therapies, and combination regimens to identify potential treatments for chemoresistant ovarian cancer. Moreover, it serves as a tool for studying tumor-microenvironment interactions and the processes involved in metastasis, particularly to the peritoneal cavity. OAW-28 remains a valuable resource for advancing understanding and treatment strategies in ovarian cancer research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.