SK-LU-1

SK-LU-1 is a human lung adenocarcinoma cell line widely used in cancer research, particularly in studies focused on non-small cell lung cancer (NSCLC). As a cisplatin-sensitive cell line, SK-LU-1 is often employed in studies evaluating chemotherapy resistance, cancer cell cycle progression, and apoptosis mechanisms. One of the defining features of SK-LU-1 is its utility in assessing the cytotoxic effects of various anti-cancer compounds, including those that modulate the cell cycle or induce apoptosis through targeted therapies. For instance, certain 6-substituted imidazopyridine derivatives have been shown to induce G2/M phase arrest and apoptosis in SK-LU-1 cells, indicating that these compounds may inhibit cyclin-dependent kinases (CDKs) involved in cancer cell division.

Additionally, SK-LU-1 cells have been used in studies exploring the immunomodulatory effects of agents like melatonin. In co-culture experiments with peripheral blood mononuclear cells (PBMCs), melatonin was shown to enhance the immune system's ability to induce apoptosis in SK-LU-1 cells. The treatment led to increased oxidative stress, reduced glutathione (GSH) levels, and cell cycle arrest at the G0/G1 phase, suggesting that melatonin may have potential as a supplementary treatment in NSCLC by boosting immune response and promoting cancer cell death.

Overall, SK-LU-1 provides a robust in vitro model for studying lung adenocarcinoma and testing novel therapeutic agents, including those that target the cell cycle, induce apoptosis, or modulate immune responses. Its responsiveness to chemotherapeutic agents like cisplatin and the wide range of experimental data available make it an important tool in NSCLC research.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.