MFE-280

The MFE-280 cell line is a human-derived endometrial adenocarcinoma cell line, specifically representative of a poorly differentiated (Grade 3) endometrial carcinoma. This cell line is extensively used in gynecological cancer research to model the behavior of aggressive endometrial cancers, particularly those associated with poor prognosis. MFE-280 cells exhibit low or undetectable expression of estrogen receptor (ER) isoforms, distinguishing them from less aggressive endometrial cancer cell lines, such as Ishikawa cells. This feature makes them an ideal model for studying ER-independent mechanisms of endometrial cancer progression.

These cells are utilized to explore the role of molecular and cellular pathways involved in cancer proliferation, invasion, and metastasis. For example, MFE-280 cells have been used to evaluate the effects of leptin, a hormone linked to obesity, which is a significant risk factor for endometrial cancer. Leptin promotes epithelial-mesenchymal transition (EMT) and enhances proliferation and invasion in MFE-280 cells through activation of the JAK2/STAT3 signaling pathway, underscoring its importance in endometrial cancer pathogenesis. Additionally, pharmacological inhibitors targeting aquaporin-1 (AQP1) ion channels have demonstrated effectiveness in reducing the invasive potential of MFE-280 cells, highlighting potential therapeutic approaches for aggressive endometrial cancers.

Therapeutic research employing MFE-280 cells has also examined selective Liver X receptor (LXR) agonists, such as GW3965, which have been shown to significantly reduce the proliferation of these cells. This aligns with their utility in identifying novel drug targets and evaluating therapeutic compounds aimed at advanced and treatment-resistant endometrial cancers. The MFE-280 cell line continues to be a valuable resource for dissecting the mechanisms underlying aggressive endometrial cancer and for advancing therapeutic strategies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.