ID8

The ID8 cell line is a widely utilized murine model derived from spontaneous transformation of C57BL/6 mouse ovarian surface epithelial (MOSE) cells. This cell line closely mimics human epithelial ovarian cancer, making it a vital tool for preclinical research into ovarian cancer pathophysiology and treatment. ID8 cells are known for their ability to grow intraperitoneally in immunocompetent C57BL/6 mice, facilitating studies of tumor progression and metastasis. This model is particularly relevant for examining peritoneal tumor formation and ascites development, which are key features of advanced ovarian cancer in patients.

ID8 cells exhibit the capability to form tumors when injected intraperitoneally, leading to disseminated cancer throughout the abdominal cavity and ascitic fluid accumulation. These properties enable the exploration of tumor-host interactions, including the role of the immune system and tumor microenvironment in cancer progression. In studies involving immunotherapies or combined treatment approaches, ID8 has proven valuable for evaluating the effects of interventions such as chemotherapy agents like carboplatin and immune checkpoint inhibitors targeting PD-L1.

Research involving ID8 models has shown their utility in examining the influence of tumor metabolism on immune cell behavior, particularly macrophage polarization and function. For instance, tumors induced by ID8 cells can modulate the metabolism of peritoneal macrophages, altering their oxidative phosphorylation (OXPHOS) and promoting tumor growth through metabolic crosstalk. These insights have paved the way for exploring targeted metabolic therapies that may inhibit tumor-promoting immune cell adaptations.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.