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B-LCL-HROC285
Tissue
外周血
Disease
腺癌
Metastatic site
不适用（来自林奇综合征结直肠癌患者的EBV转化B细胞淋巴细胞样细胞）
Synonyms
B-LCL CO285, Bc HROC285

Documents

B-LCL-HROC285

Cell line · Product number 300869

B-LCL-HROC285 is an Epstein-Barr virus (EBV) transformed B lymphocyte cell line derived from a patient, who had colon adenocarcinoma associated with Lynch...

US$800.00excl. tax and shipping
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Lot
Recommended (freshest lot)
1

01General information

Description

B-LCL-HROC285是一种由爱泼斯坦-巴氏病毒（EBV）转化的B淋巴细胞系，其来源是一名患有林奇综合征结肠腺癌的患者。这种特殊类型的结肠癌与遗传性非息肉病结直肠癌（HNPCC）有关，通常由 DNA 错配修复基因突变引起。利用 B-LCL-HROC285 细胞系可以研究 B 细胞中与 EBV 相关的转化过程，并深入了解与癌症相关的免疫反应。

B-LCL-HROC285 细胞系是了解免疫系统与癌细胞相互作用的重要工具，尤其是了解转化的 B 细胞如何与林奇综合征引起的结直肠癌中的免疫环境相互作用。由于其遗传背景和 EBV 转化过程会影响 B 细胞的增殖和克隆选择，因此该细胞系对免疫学和肿瘤学研究非常有用。

Tissue
外周血
Disease
腺癌
Metastatic site
不适用（来自林奇综合征结直肠癌患者的EBV转化B细胞淋巴细胞样细胞）
Applications
T细胞和自然杀伤细胞（NK细胞）检测；HLA分型；林奇综合征免疫学；错配修复（MMR）缺陷相关的免疫应答；细胞毒性T淋巴细胞（CTL）检测靶细胞；HROC生物样本库患者配对研究
Synonyms
  • B-LCL CO285
  • Bc HROC285

02Classification

Organism
Human
Sex
Female
Growth properties
Suspension
GMO status
GMO-S2
GMO status details
GMO-S2: This B-LCL contains a stably maintained EBV episome (EBNA-1/-2/-3, LMP-1/-2). EBV is classified as risk group 2; BSL-2 containment required. This classification applies within Germany; regulations may differ elsewhere.
Biosafety level
2

03Characteristics

Morphology
圆形电池
Cell type
B 淋巴细胞

04Identifiers

Citation
B-LCL-HROC285（Cytion 目录号 300869）
NCBI TaxID
9606
Cellosaurus
Not assigned

05Expression and susceptibility

Viruses
变形体：EBV

06Handling and culture

Culture medium
RPMI 1640，w：2.0 mM 稳定谷氨酰胺，w：2.0 g/L NaHCO3（Cytion 文章编号 820700a）。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% 热灭活 FBS
Subculturing
通过上下吸取操作轻柔匀化烧瓶中的细胞悬液，随后取代表性样本测定每毫升细胞密度。用新鲜培养基将悬液稀释至1×10⁵细胞/毫升浓度，并将调整后的悬液分装至新烧瓶中进行后续培养。
Thawing and culturing

  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基（包括 FBS）+10% DMSO 或 CM-1（Cytion 商品目录编号 800100）作为冷冻保存培养基，以获得足够的解冻后存活率，CM-1（Cytion 商品目录编号 800100）含有优化的渗透保护剂和代谢稳定剂，可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
30年
Ethnicity
高加索人