P388

P388 is a murine lymphoid neoplasm cell line derived from a spontaneous lymphocytic leukemia in DBA/2 mice. It is commonly used in cancer research, particularly for studying leukemia and testing anti-cancer compounds. P388 cells grow in suspension and exhibit a doubling time of approximately 24 hours under optimal culture conditions. The cells are characterized by their rapid proliferation and high sensitivity to chemotherapeutic agents, making them a valuable tool for evaluating the efficacy of new cancer treatments.

P388 cells express typical markers of lymphoid lineage, including surface immunoglobulins and various cell surface antigens associated with B-cells. Researchers often utilize this cell line in in vivo models by inoculating mice to study tumor growth, metastasis, and response to therapies. Additionally, the P388 cell line serves as a model for investigating the molecular mechanisms underlying leukemia, such as the role of specific oncogenes and tumor suppressor genes.

Despite its widespread use, the P388 cell line has limitations, such as the lack of human relevance and potential genetic drift over extended culture periods. Therefore, researchers often complement studies involving P388 cells with other models to obtain a comprehensive understanding of leukemia biology and treatment responses.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.