U2OS

U2OS cells, an osteosarcoma cell line derived from a human osteosarcoma patient, play a significant role in cancer research, particularly in the study of bone cancer. U2OS cells are used extensively in cancer research, drug development, apoptosis studies, genetic research, and radiation oncology studies. The value of U2OS cells lies in their application to investigate apoptosis and drug resistance, essential for creating small molecule inhibitors and similar therapeutic agents.

In the realm of clinical osteosarcoma research, the U2OS cell line is instrumental in examining biological responses to radiotherapy, thereby enriching our understanding of osteosarcoma biology. These cells are also pivotal in investigating chromatin modifications and their impact on cell biology, especially in the context of tumor formation and cancer progression.

The U2OS cell line, also referred to as the OS cell line, is recognized for its in vivo tumor formation capacity when administered through subcutaneous and intramuscular injections. The tumors produced by U2OS cells are characterized as high-grade sarcomas and exhibit significant osteoid production, which is a hallmark of osteosarcoma. Additionally, these tumors showed infiltration by immune cells. U2OS therefore serves as a representative model for studying human osteosarcoma, its interactions with the human immune system and tumor immunology. One of the challenges, however, is ensuring the osteosarcoma U2OS cell line accurately reflects the tumors in vivo, given the variability in tumor formation capacity.

In summary, sarcoma cell lines such as U2OS serve as a pivotal tool in understanding osteosarcoma, offering valuable insights into cancer biology, therapeutic development, and the complexities of tumor-immune system interactions, while highlighting the need for accurate in vivo tumor modeling.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.