HBZY-1

HBZY-1 cells are primary cells isolated from the glomerulus of rat kidneys, specifically from mesangial cells. These cells are highly regarded in scientific research due to their origin and functionality. The glomerulus, a key structure in the kidney, is crucial for blood filtration and purification. Mesangial cells play a significant role in maintaining the structure and function of this specialized renal unit. Thus, HBZY-1 cells provide a valuable model for studying the intricacies of renal biology and advancing our understanding of kidney-related diseases.

Employed in various scientific studies, HBZY-1 cells allow researchers to delve into mesangial cell function and the pathogenesis of kidney diseases. This makes them an essential tool for investigating cellular processes, signaling pathways, and molecular interactions that are pivotal in renal biology. Utilizing these cells in vitro offers insights into the molecular mechanisms governing mesangial cell behavior, enhancing our knowledge of their role in kidney function and disease.

Furthermore, HBZY-1 cells are utilized in pathophysiological studies of kidney diseases, such as glomerulonephritis and diabetic nephropathy. These cells can be subjected to experimental conditions that mimic disease states, providing a platform to study the molecular events that contribute to renal pathology. This capacity makes HBZY-1 cells instrumental in drug discovery and the development of therapeutic interventions aimed at treating kidney-related disorders, potentially leading to significant advancements in patient care and treatment strategies.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.