HET-1A

The HET-1A cell line is derived from the human esophageal epithelium and is used extensively in gastroenterological research. These cells provide a valuable model for studying the physiology and pathology of the esophagus, particularly in the context of esophageal diseases such as Barrett's esophagus and esophageal cancer. HET-1A cells are often employed to investigate the cellular responses to various environmental and dietary factors that may contribute to esophageal disease development and progression.

HET-1A cells exhibit an epithelial morphology and retain characteristics typical of esophageal epithelial cells, including the expression of cytokeratins and other epithelial markers. They are used in studies focusing on epithelial cell biology, differentiation, and the mechanisms of cellular transformation. Researchers utilize HET-1A cells to explore the effects of acid and bile reflux, oxidative stress, and inflammation on esophageal cells, providing insights into the pathophysiology of gastroesophageal reflux disease (GERD) and its potential progression to Barrett's esophagus or esophageal adenocarcinoma. Additionally, HET-1A cells are used to assess the impact of various chemopreventive and therapeutic agents on esophageal epithelial health, making them an important tool for advancing the understanding and treatment of esophageal disorders.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.