UM-SCC-112

UM-SCC-112 is a human head and neck squamous cell carcinoma (HNSCC) cell line established at the University of Michigan Head and Neck Oncology laboratory as part of the UM-SCC panel. The UM-SCC series represents one of the most extensively characterized and widely distributed HNSCC cell line collections in the world, derived from patients with oral cavity, laryngeal, hypopharyngeal, and oropharyngeal squamous cell carcinomas treated at the University of Michigan Medical Center. UM-SCC-112 grows as an adherent monolayer with epithelial-like morphology and is used as part of a multi-line panel for comprehensive preclinical characterization of HNSCC biology.

UM-SCC-112 is applicable in studies of HNSCC biology, drug sensitivity and resistance, radiation response, EGFR and other signalling pathway analyses, and comparative molecular characterization within the UM-SCC panel. The line is suitable for pharmacological assays, invasion and migration studies, and xenograft models in immunocompromised hosts. Site-specific and donor-specific details for UM-SCC-112 should be verified from the University of Michigan SPORE Tissue Core for the most current annotations.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.