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UM-SCC-112
Tissue
頭頸部、口腔
Disease
頭頸部鱗狀細胞癌(HNSCC)

Documents

UM-SCC-112

Cell line · Product number 305720

UM-SCC-112 is a human head and neck squamous cell carcinoma (HNSCC) cell line established at the University of Michigan Head and Neck Oncology laboratory ...

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1

01General information

Description

UM-SCC-112 是一種人類頭頸部鱗狀細胞癌(HNSCC)細胞系,由密西根大學頭頸部腫瘤學實驗室建立,隸屬於 UM-SCC 細胞系系列。 UM-SCC 系列是全球中表徵最詳盡且分發最廣泛的人類頭頸部鱗狀細胞癌細胞系集合之一,源自於在密西根大學醫學中心接受治療的口腔、喉部、下咽部及口咽部鱗狀細胞癌患者。 UM-SCC-112 以黏附單層形式生長,呈現上皮樣形態,並作為多株系組合的一部分,用於對頭頸部鱗狀細胞癌(HNSCC)生物學進行全面的臨床前特徵分析。

UM-SCC-112 適用於頭頸部鱗狀細胞癌(HNSCC)生物學、藥物敏感性與抗藥性、放射反應、EGFR 及其他訊號傳導路徑分析,以及在 UM-SCC 細胞系組合內的比較分子特徵分析。該細胞系適用於藥理學檢測、侵襲與遷移研究,以及免疫缺陷宿主中的異種移植模型。 有關 UM-SCC-112 的部位特異性及捐贈者特異性詳細資訊,應向密西根大學 SPORE 組織核心中心核實,以取得最新註解。

Tissue
頭頸部、口腔
Disease
頭頸部鱗狀細胞癌(HNSCC)
Applications
頭頸部鱗狀細胞癌(HNSCC)研究;藥物敏感性與抗藥性;放射線反應;EGFR 訊號傳導;UM-SCC 基因檢測面板研究;侵襲與遷移實驗;異種移植模型

02Classification

Organism
Human
Sex
Unspecified
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮樣
Cell type
上皮細胞
Doubling time
約 24 至 48 小時

04Identifiers

Citation
UM-SCC-112(Cytion 型號 305720)
NCBI TaxID
9606

05Handling and culture

Culture medium
DMEM,含 4.5 g/L 葡萄糖、4 mM L-谷氨醯胺、3.7 g/L NaHCO₃ 及 1.0 mM 丙酮酸鈉(Cytion 產品編號 820300a)
Incubation atmosphere

37°C,5% CO₂,加濕環境。

Supplements
在培養基中加入 10% 的胎牛血清(FBS)及 1% 的非必需胺基酸(NEAA)
Dissociation reagent
Accutase
Subculturing
移除培養基,用不含鈣和鎂的PBS清洗,加入Accutase覆蓋,於室溫下孵育 8–10 分鐘,重新懸浮於培養基中,以 300×g 離心 3 分鐘,棄去上清液,再將細胞接種於新鮮培養基中。
Split ratio
1 至 5
Seeding density
1 至 3 × 10⁴ 個細胞/cm²
Fluid renewal
每 2 至 3 天
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

  7. Follow the procedure described under Post-Thaw Recovery

06Quality control and cryopreservation

Freeze medium
作為冷凍保存培養基,我們使用完全生長培養基加上 10% 的二甲基亞砜(DMSO),以確保解凍後的細胞存活率達到適當水準。
Storage conditions

若需長期保存,請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前,可將試管暫時存放於 −80 °C 環境中,作為短期過渡步驟。

Shipping conditions

冷凍保存的細胞系會以乾冰冷藏,並裝於經驗證的隔熱包裝中,包裝內含有足夠的冷媒,以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後,請立即檢查容器,並盡快將試管轉移至適當的儲存場所。

07Donor

Donor age
年齡不詳
Ethnicity
未指定族裔