WEHI-3B

The WEHI-3B cell line is a murine leukemia cell line that is extensively utilized as a model for studying myelomonocytic differentiation and the pathophysiology of leukemia. Originally derived from BALB/c mice, these cells exhibit characteristics of myeloid progenitor cells and have been instrumental in the research of hematopoietic differentiation and regulation. The WEHI-3B line is particularly important for studies related to the influence of growth factors on leukemic cells and has been used to evaluate the hematopoietic activity of various substances including colony-stimulating factors.

This cell line is not only significant for its use in leukemia research but also serves as a tool in the study of macrophage and granulocyte function, thanks to its ability to differentiate into these cell types under certain experimental conditions. Studies using WEHI-3B cells have contributed to a better understanding of the molecular pathways involved in cell differentiation and the impact of genetic alterations on leukemia progression. Furthermore, the WEHI-3B cell line is used in testing the biological activity of monocytic colony-stimulating factor (M-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF), highlighting its versatility and utility in hematological research contexts.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.