CLS-CD-3575

CLS-CD-3575 is a human cancer cell line included in curated cell line collections for oncological research. It is derived from a solid tumor of epithelial origin obtained from an adult patient and has been adapted to continuous in vitro culture. The cells grow adherently under standard culture conditions and exhibit morphology consistent with their tissue of origin, forming monolayers with epithelial-like characteristics. As with many established carcinoma cell lines, CLS-CD-3575 demonstrates stable proliferation and suitability for routine passaging.

Molecularly, CLS-CD-3575 displays genomic alterations typical of malignant epithelial tumors, including chromosomal imbalances and deregulated signaling pathways associated with proliferation and survival. Depending on the specific tumor origin, expression of lineage-associated cytokeratins and tumor-related markers may be detected. Such features make the line suitable for studies of oncogenic signaling, cell cycle regulation, apoptosis, and drug response profiling in vitro.

CLS-CD-3575 is used in experimental settings including cytotoxicity testing, molecular pathway analysis, and evaluation of targeted therapeutic strategies. Its reproducible growth characteristics and compatibility with standard biochemical, molecular biology, and imaging techniques make it a practical model for mechanistic cancer research and preclinical compound screening.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.