HEK293FT

The HEK293FT cell line is a derivative of the HEK293 cell line, originally derived from human embryonic kidney cells. The "FT" designation indicates that these cells have been transfected with the SV40 large T-antigen gene, which enhances their ability to replicate plasmid vectors containing the SV40 origin of replication. This modification makes 293FT cells particularly useful for high-efficiency production of viral vectors, such as lentiviruses and adenoviruses, and for transfection studies in molecular biology and gene therapy research.

HEK293FT cells exhibit an epithelial morphology and grow rapidly in culture, providing a robust and reliable system for producing high-titer viral stocks. They retain many of the characteristics of the parental HEK293 cells, including high transfection efficiency and the ability to support the replication of recombinant viruses. Researchers utilize 293FT cells to produce viral vectors for gene delivery, to study gene function and regulation, and to develop gene therapies for various diseases. Their role in the production of viral vectors makes 293FT cells a cornerstone in the fields of gene therapy, functional genomics, and molecular cloning, facilitating the advancement of research and therapeutic development.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.