HEK293 suspension-adapted

The HEK293 suspension-adapted cell line is a variant of the human embryonic kidney 293 (HEK293) cells that has been modified to grow in suspension culture rather than adherent culture. This adaptation is significant for industrial applications where large-scale protein production is required. The cells maintain many of the characteristics of the original HEK293 line, including a robust transient transfection efficiency and the ability to post-translationally modify expressed proteins in a manner similar to that of native human cells.

These cells are particularly valued in the biotechnology and pharmaceutical industries for the production of recombinant proteins and viruses for gene therapy and vaccine development. The adaptation to suspension culture allows for easier scalability and simplifies the harvesting process, making it more suitable for commercial-scale bioprocessing. The HEK293 suspension-adapted cell line supports various viral production systems, including adenovirus, lentivirus, and adeno-associated virus (AAV), which are pivotal in therapeutic applications and research.

Overall, the HEK293 suspension-adapted cell line is a crucial tool in the fields of molecular biology and bioprocessing, providing a versatile platform for the production of various biologically active molecules. Its ease of genetic manipulation and ability to produce proteins that are correctly folded and post-translationally modified according to human cell patterns make it an indispensable resource in many advanced therapeutic and research settings.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium.

7. Follow the procedure described under Post-Thaw Recovery

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.