IGR-1 细胞

IGR-1 细胞系来源于人类恶性黑色素瘤，是研究黑色素瘤病理生理学和测试抗癌疗法的宝贵模型。这些细胞是上皮细胞，具有侵袭性黑色素瘤的典型特征，包括快速增殖和在软琼脂中形成菌落的能力，这是致癌转化的标志。IGR-1 细胞系特别适用于了解黑色素瘤进展的分子机制的研究，以及靶向疗法和免疫疗法的开发和测试。

IGR-1细胞携带黑色素瘤常见的突变，包括MAPK/ERK通路的改变，这种通路在这种癌症类型中经常失调。这些突变导致细胞系能够不受控制地增殖并抵抗细胞凋亡。研究人员利用 IGR-1 细胞来研究各种抑制剂对该信号通路的影响，从而为潜在的治疗策略提供启示。此外，该细胞系表达黑色素瘤相关抗原，因此适合研究针对黑色素瘤的免疫反应，包括开发新型免疫治疗方法。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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