SNU-5

SNU-5细胞系是由转移病灶建立的人胃癌模型，其分子异常特征已被确证，尤其涉及p53抑癌基因的突变。 研究表明，通过Northern印迹分析检测到p53 mRNA缺失，证实SNU-5存在p53基因转录本缺失。核糖核酸酶保护实验和测序进一步证实：该细胞系编码区未检测到可测突变，但完全无法表达转录本，表明基因沉默可能源于调控或表观遗传机制，而非结构性突变。

蛋白质组学分析为深入理解SNU-5的分子特征提供了新视角。大规模研究将SNU-5纳入人类癌细胞系蛋白质组测绘的细胞系面板。 在此背景下，SNU-5为整合数千种蛋白质质谱定量数据的分析集提供了重要贡献。这些蛋白质组学数据集已与转录组、基因组及表型特征建立关联，全面呈现了蛋白质表达、转录后调控及药物响应特性。此类数据集使SNU-5成为研究胃癌生物学机制的宝贵模型，尤其在转移性疾病和p53通路失调领域具有重要价值。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染，每天都要对细胞培养物进行目视检查。

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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