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SNU-601
Tissue
胃部
Disease
胃标志环细胞腺癌
Metastatic site
腹水
Synonyms
SNU601, NCI-SNU-601

Documents

SNU-601

Cell line · Product number 305282

US$550.00excl. tax and shipping
In stock
Lot
Recommended (freshest lot)
  • CoA
1

01General information

Description

SNU-601 细胞系来源于分化较差的人类胃癌,被广泛用于胃癌研究。胃腺癌是一种常见的侵袭性胃癌,该细胞系是研究胃腺癌分子和细胞机制的重要模型。SNU-601 细胞对于研究与胃癌相关的基因和表观遗传学改变以及测试潜在治疗药物的疗效非常有价值。

SNU-601 细胞呈现上皮形态,并表达胃癌的特征标记,包括细胞角蛋白和癌胚抗原(CEA)。它们携带胃癌中常见的基因改变,如癌基因和肿瘤抑制基因(如 TP53)的突变。研究人员利用 SNU-601 细胞探索胃癌进展过程中的关键信号通路,如 PI3K/Akt、Wnt/β-catenin 和 MAPK 通路。这些细胞还被用于化疗药物、靶向疗法和联合疗法的高通量药物筛选试验和临床前测试。此外,SNU-601 细胞还被用于研究耐药性机制和开发克服耐药性的策略。SNU-601细胞系与胃癌研究的相关性凸显了它在促进我们对这种恶性肿瘤的了解以及为胃癌患者开发更有效的治疗方法方面的重要性。

Tissue
胃部
Disease
胃标志环细胞腺癌
Metastatic site
腹水
Synonyms
  • SNU601
  • NCI-SNU-601

02Classification

Organism
Human
Sex
Male
Growth properties
Adherent
Biosafety level
1

03Characteristics

Morphology
上皮细胞

04Identifiers

Citation
SNU-601(Cytion 目录号 305282)
NCBI TaxID
9606
Cellosaurus
CVCL_0101

05Expression and susceptibility

Mutational profile
突变:KRAS,p.Gly12Asp (c.35G>A),杂合;突变:PIK3CA,p.Glu542Lys(c.1624G>A),杂合;突变:TP53,p.Arg273His(c.818G>A),杂合子

06Handling and culture

Culture medium
RPMI 1640,w:2.0 mM 稳定谷氨酰胺,w:2.0 g/L NaHCO3(Cytion 文章编号 820700a)。
Incubation atmosphere

37°C, 5%CO2, 加湿环境。

Supplements
在培养基中添加 10% FBS、25 mM HEPES
Dissociation reagent
Accutase
Subculturing
去除粘附细胞上的旧培养基,用不含钙和镁的 PBS 冲洗。T25 烧瓶用 3-5 毫升 PBS,T75 烧瓶用 5-10 毫升。然后用 Accutase 完全覆盖细胞,T25 烧瓶用 1-2 毫升,T75 烧瓶用 2.5 毫升。让细胞在室温下孵育 8-10 分钟,使其分离。孵育后,用 10 毫升培养基轻轻混合细胞使其重悬,然后用 300xg 离心 3 分钟。弃去上清液,用新鲜培养基重悬细胞,并将其转移到已装有新鲜培养基的新烧瓶中。
Thawing and culturing
  1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

  2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

  3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

  4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

  5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

  6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

  7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

  8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

07Quality control and cryopreservation

Sterility

使用基于 PCR 的检测方法和基于发光的支原体检测方法排除支原体污染。

为确保没有细菌、真菌或酵母菌污染,每天都要对细胞培养物进行目视检查。

Freeze medium
我们使用完全生长培养基(包括 FBS)+10% DMSO 或 CM-1(Cytion 商品目录编号 800100)作为冷冻保存培养基,以获得足够的解冻后存活率,CM-1(Cytion 商品目录编号 800100)含有优化的渗透保护剂和代谢稳定剂,可提高恢复能力并减少冷冻引起的应激。
Storage conditions

如需长期保存,可将样品瓶放入气相液氮中,温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

Shipping conditions

冷冻保存的细胞系用干冰装运,并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后,应立即检查容器,并立即将小瓶转移到适当的储藏室。

08Donor

Donor age
34 年
Ethnicity
东亚

10Product details

Composition table