5637

5637 is a bladder carcinoma cell line isolated from the urinary bladder of a 68-year-old man with grade II carcinoma. 5637 cells produce and secrete several growth factors, such as SCF, IL-1, IL-6, G-CSF, and GM-CSF. These cytokines are functionally active and can be a valuable source for the culture of growth factor-responsive or dependent hematopoietic primary cells and cell lines.

The karyotype modal chromosome number of 5637 cells is 67, ranging from 59 to 71. The stemline modal chromosome number is 67 at 36% and polyploidy at 0.6%. Fourteen marker chromosomes are common to these cells, including 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q). Additional markers, like der(5)t(5;7)(q31;p11) and 1p, were only found specific to a minor subpopulation, as well as microchromosomes and double minutes (DM). Some cells include one or occasionally two Y chromosomes.

5637 cells are tumorigenic and have been shown to induce tumours in nude mice inoculated subcutaneously. The doubling time of 5637 cells is approximately 24 hours. The isoenzyme profile of 5637 cells consists of isoform 1 of AK-1, ES-D, Me-2 and PGM1, isoform 1 and 2 of GLO-I, isoform B of G6PD, as well as isoform 2 of PGM3. In terms of oncogenes, 5637 cells are positive for FGFR3, PIK3CA, HRAS, KRAS, NRAS, TERT, and CDKN2A but negative for TP53 and belong to the molecular bladder cancer subtype l5637 is a bladder carcinoma cell line isolated from the urinary bladder of a 68-year-old man with grade II carcinoma. 5637 cells produce and secrete several growth factors, such as SCF, IL-1, IL-6, G-CSF, and GM-CSF. These cytokines are functionally active and can be a valuable source for the culture of growth factor-responsive or dependent hematopoietic primary cells and cell lines.

The karyotype modal chromosome number of 5637 cells is 67, ranging from 59 to 71. The stemline modal chromosome number is 67 at 36% and polyploidy at 0.6%. Fourteen marker chromosomes are common to these cells, including 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q). Additional markers, like der(5)t(5;7)(q31;p11) and 1p, were only found specific to a minor subpopulation, as well as microchromosomes and double minutes (DM). Some cells include one or occasionally two Y chromosomes.

5637 cells are tumorigenic and have been shown to induce tumours in nude mice inoculated subcutaneously. The doubling time of 5637 cells is approximately 24 hours. The isoenzyme profile of 5637 cells consists of isoform 1 of AK-1, ES-D, Me-2 and PGM1, isoform 1 and 2 of GLO-I, isoform B of G6PD, as well as isoform 2 of PGM3.

In terms of oncogenes, 5637 cells are positive for FGFR3, PIK3CA, HRAS, KRAS, NRAS, TERT, and CDKN2A but negative for TP53 and belong to the molecular bladder cancer subtype luminal. In conclusion, 5637 cells are a valuable tool for cancer research, especially with respect to the study of growth factors, cell division, oncogenes, and bladder cancer.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

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