CHO-CD36

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CHO-CD36 cells are recombinant Chinese hamster ovary (CHO) cells engineered to stably express human CD36, a multifunctional class B scavenger receptor also known as platelet glycoprotein IV (GPIV) or fatty acid translocase (FAT). CD36 is broadly involved in lipid uptake, fatty acid metabolism, angiogenesis, inflammation, innate immunity, and cell adhesion. The receptor interacts with a wide range of ligands, including oxidized low-density lipoproteins (oxLDL), long-chain fatty acids, thrombospondin-1, phospholipids, and apoptotic cells. Dysregulated CD36 expression has been implicated in metabolic disorders, atherosclerosis, chronic inflammation, and tumor progression, making recombinant CD36-expressing cell models valuable tools for mechanistic and therapeutic research.

CHO-CD36 cells are widely used for studying receptor-ligand interactions, lipid transport mechanisms, and therapeutic targeting of CD36-associated pathways. These cells support quantitative analysis of ligand binding, receptor internalization, fatty acid uptake, and downstream signaling events linked to oxidative stress, immune modulation, and metabolic adaptation. In oncology research, CHO-CD36 models are useful for investigating the role of CD36 in metastasis, tumor lipid metabolism, and resistance to metabolic stress. The cells are also applied in development and characterization of monoclonal antibodies, small-molecule inhibitors, lipid-targeting therapeutics, and imaging agents directed against CD36. Flow cytometry assays, uptake assays, and high-throughput screening platforms commonly utilize CHO-CD36 cells because of their stable and controlled recombinant receptor expression.

For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)

For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)

37°C, 5% CO₂, humidified atmosphere.

After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.