HS-695T

The HS-695T cell line is derived from human melanoma, a type of skin cancer characterized by the malignant transformation of melanocytes. These cells were originally obtained from an adult patient and have since been extensively utilized in research focused on melanoma biology, tumorigenesis, and cancer metastasis. The HS-695T cell line exhibits key characteristics of melanoma, including the ability to proliferate rapidly and form tumors when transplanted into immunocompromised mice. This cell line retains many of the molecular and genetic features of the original tumor, making it a valuable model for studying the underlying mechanisms of melanoma progression and for testing potential therapeutic agents.

HS-695T cells express various melanoma-associated markers, including Melan-A, tyrosinase, and HMB-45, which are commonly used to identify and study melanocytic tumors. These cells are also known to have mutations in genes such as BRAF and NRAS, which are frequently observed in melanoma and contribute to the oncogenic signaling pathways driving tumor growth and survival. Researchers use the HS-695T cell line to explore the effects of targeted therapies, including BRAF and MEK inhibitors, and to investigate the development of resistance to these treatments. Overall, the HS-695T cell line is a critical tool in melanoma research, aiding in the discovery of new therapeutic strategies and improving our understanding of this aggressive cancer.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.