Human Mesenchymal Stem Cells, Umbilical Cord - Artery

Human Mesenchymal Stem Cells (hMSCs) derived from the umbilical cord artery are a distinct and promising subtype of mesenchymal stem cells, offering several unique advantages over other MSC sources. Unlike MSCs derived from bone marrow or adipose tissue, umbilical cord artery MSCs are harvested from a more primitive and less invasive source, providing a younger and potentially more potent cell population. This origin confers a higher proliferative capacity and longer telomeres, which may enhance their self-renewal abilities and reduce the risk of senescence during extended culture. Furthermore, MSCs from the umbilical cord artery typically express a unique set of surface markers and have a lower immunogenic profile, making them particularly suitable for allogeneic applications and reducing the risk of immune rejection.

In vitro, umbilical cord artery-derived MSCs demonstrate robust multipotency, with the ability to differentiate into adipocytes, osteoblasts, and chondrocytes when exposed to specific differentiation media. This versatility is comparable to that of MSCs derived from other tissues, but with the added benefit of their primitive nature, which may enhance their therapeutic potential. Each batch of these MSCs undergoes stringent quality control, including assessments for viability, purity, and potency, ensuring that the cells meet high standards for research applications. The cells are cryopreserved at early passages using a specialized cryomedium, maintaining their high viability (92% to 95%) upon thawing, which is crucial for their effective use in downstream applications.

Overall, hMSCs from the umbilical cord artery offer a combination of easy accessibility, high proliferative capacity, and low immunogenicity, making them a valuable tool for a wide range of research studies, particularly those focusing on regenerative medicine and immune modulation. These cells, harvested with full donor consent, represent a high-quality and ethically sourced option for researchers looking to explore the full potential of mesenchymal stem cells in vitro.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.