MA-891

MA-891 is a highly metastatic murine mammary gland carcinoma cell line, established from a spontaneous mammary tumor in a mouse and selected for its aggressive metastatic behavior. The line grows as an adherent monolayer and is classified as a syngeneic tumor cell line compatible with immunocompetent mouse hosts, enabling in vivo studies of mammary carcinoma growth, metastasis, and immune interaction. MA-891 represents a highly metastatic variant selected for its propensity to disseminate to distant organs, particularly the lungs.

MA-891 is applicable in murine mammary carcinoma research including studies of metastatic cascade biology, invasion, angiogenesis, tumor-immune interactions, and preclinical evaluation of anti-metastatic and anti-tumor agents. Its syngeneic origin enables testing of immunotherapy approaches in immunocompetent hosts, including checkpoint inhibitor evaluation and cancer vaccine development. The high metastatic potential makes it a useful model for studying metastasis prevention strategies and the biology of aggressive mammary carcinoma.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.