MA-891

MA-891 是一種高度轉移性的小鼠乳腺癌細胞系，源自一隻小鼠體內的自發性乳腺腫瘤，並因其具侵略性的轉移行為而經篩選建立。 該細胞系以黏附性單層形式生長，被歸類為與免疫功能正常小鼠宿主相容的同基因腫瘤細胞系，可藉此進行乳腺癌生長、轉移及免疫相互作用的體內研究。 MA-891 是一種高度轉移性的變異株，因其易於擴散至遠端器官（尤其是肺部）而被篩選出來。

MA-891 適用於小鼠乳腺癌研究，包括轉移級聯生物學、侵襲、血管生成、腫瘤-免疫相互作用的研究，以及抗轉移和抗腫瘤藥物的臨床前評估。 其同基因源特性，使其能夠在免疫功能正常的宿主中測試免疫療法，包括檢查點抑制劑的評估及癌症疫苗的開發。其高度的轉移潛力，使其成為研究轉移預防策略及侵襲性乳腺癌生物學的有用模型。

37°C，5% CO_₂，加濕環境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

若需長期保存，請將試管置於溫度約為 −150 至 −196 °C 的氣相液氮中。僅在轉移至液氮之前，可將試管暫時存放於 −80 °C 環境中，作為短期過渡步驟。

冷凍保存的細胞系會以乾冰冷藏，並裝於經驗證的隔熱包裝中，包裝內含有足夠的冷媒，以確保在運輸過程中溫度維持在約 −78 °C。收到貨物後，請立即檢查容器，並盡快將試管轉移至適當的儲存場所。

在 Cytion，我們對產品的品質與存活率提供保障。 我們保證，自本公司設施出貨後 60 天內，我們的細胞在培養開始時仍具存活能力。 我們保證所有材料均符合 分析證明書及／或產品說明書中所載的規格。

詳情請參閱我們的 《保固聲明》。