MA-891

MA-891是一种高度转移性的小鼠乳腺癌细胞系，由小鼠自发性乳腺肿瘤建立，并因其具有侵袭性转移特征而被筛选出来。 该细胞系以贴壁单层形式生长，被归类为与免疫功能正常的鼠宿主相容的同基因肿瘤细胞系，可用于研究乳腺癌的生长、转移及免疫相互作用。 MA-891 是一种高度转移性变异株，因其易向远端器官（尤其是肺部）扩散而被筛选出来。

MA-891 适用于小鼠乳腺癌研究，包括转移级联生物学、侵袭、血管生成、肿瘤-免疫相互作用的研究，以及抗转移和抗肿瘤药物的临床前评估。 其同基因来源使其能够在免疫功能正常的宿主中测试免疫疗法，包括检查点抑制剂的评估和癌症疫苗的开发。其高转移潜力使其成为研究转移预防策略和侵袭性乳腺癌生物学的有用模型。

37°C, 5%_CO2, 加湿环境。

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

如需长期保存，可将样品瓶放入气相液氮中，温度约为 -150 至 -196 ℃。在-80 °C下保存只能作为转入液氮前的短暂过渡。

冷冻保存的细胞系用干冰装运，并用经过验证的隔热包装和足够的制冷剂在整个运输过程中保持约 -78 °C 的温度。收到货后，应立即检查容器，并立即将小瓶转移到适当的储藏室。

在Cytion，我们保证产品的质量和活力。 我们保证，从我们的工厂发货后 60 天内，我们的细胞在开始培养时都是存活的。 我们保证所有材料均符合分析证书和/或产品说明书中描述的规格。

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