NCI-H2347

The NCI-H2347 cell line is a human non-small cell lung cancer (NSCLC) cell line derived from a lung adenocarcinoma. This cell line is widely used in studies of lung cancer biology, particularly for research involving tumor suppressor gene mutations and pathways involving apoptosis, chemotherapy resistance, and viral-based cancer therapies. NCI-H2347 has wild-type p53, which contrasts with many lung cancer cell lines that harbor p53 mutations, making it a relevant model for studying the differences in therapeutic response based on p53 status.

This cell line has been utilized in experiments to test the efficacy of novel treatments such as ONYX-015, a genetically modified adenovirus that selectively replicates in and lyses tumor cells with non-functional p53. While ONYX-015 was highly effective in lung cancer cell lines with p53 mutations, such as NCI-H522, its effect on NCI-H2347, which has wild-type p53, was limited. Additionally, NCI-H2347 has been involved in studies focusing on MET signaling, particularly in relation to resistance to EGFR tyrosine kinase inhibitors (TKIs). It has been shown that while MET gene amplification is not observed in this cell line, its MET protein can still be activated by EGFR mutations, suggesting a complex interplay between MET and EGFR signaling pathways.

37°C, 5% CO₂, humidified atmosphere.

1. Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit.

2. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required.

3. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains.

4. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening.

5. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently.

6. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium.

7. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth.

8. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods.

To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.

At Cytion, we stand behind the quality and viability of our products. We guarantee that our cells will be viable at the start of culture for 60 days after shipment from our facilities. We assure that all materials conform to the specifications described in the Certificate of Analysis and/or Product Sheet.

For full details, please refer to our Warranty Statement.